ti e inverted widefield fluorescence microscope (Nikon)
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ti e inverted widefield fluorescence microscope
Ti E Inverted Widefield Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ti+e+inverted+widefield+fluorescence+microscope/Objectives/pmc12033024-584-6-5
Average 99 stars, based on 59881 article reviews
Ti E Inverted Widefield Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ti+e+inverted+widefield+fluorescence+microscope/Objectives/pmc12033024-584-6-5
Average 99 stars, based on 59881 article reviews
ti e inverted widefield fluorescence microscope - by Bioz Stars,
2026-10
99/100 stars
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other:Article Title: GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies. Article Snippet: PCRs for cloning were performed with the proof-reading polymerase ClonAmpHiFi PCR Premix (Takara) and PCR for validation of strains were performed with EmeraldAmp GT Master Mix (Takara). Article Title: High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription. Article Snippet: The cells were stained with 1:800 rabbit anti-cMyc (Cell Signaling) and 1:200 mouse anti-T. gondii (Santa Cruz) for 1 h, followed by 1:1000 goat anti-rabbit Alexa 488 (Thermo), 1:1000 goat anti-mouse Alexa 647 (Thermo), and 5 mg/mL DAPI for 1 h. For each sample, a 3x3 tiled image was acquired as above using a Nikon Plan APO 40x/0.95 objective. cMyc fluorescence intensity was quantified in ImageJ. Article Title: In vivo CRISPR screens identify GRA12 as a transcendent secreted virulence factor across Toxoplasma gondii strains and mouse subspecies Article Snippet: Black clear-bottom 96-well plates were seeded with 75,000 BMDMs per well and stimulated with 100 U/ml mIFNγ for 24 h. Cells were infected at an MOI of 3 in a media solution with 5 μg/mL propidium iodide (Thermo Fisher P3566). Fluorescence:Article Title: High-throughput identification of pathogen effector proteins that target host transcription by dual perturb-seq Article Snippet: .. Images were acquired on a Nikon Article Title: GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies Article Snippet: .. Similarly to previously established protocols , experiments in Fig. and Supplementary Fig. , were analysed on a Nikon Article Title: A heterotrimeric complex of Toxoplasma proteins promotes parasite survival in interferon gamma-stimulated human cells. Article Snippet: .. Images were acquired on a Nikon Article Title: High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription. Article Snippet: Finally, the cells were strained with 5 mg/mL DAPI plus 1:1000 goat anti-mouse Alexa 647 (Thermo) and 1:1000 goat anti-rabbit Alexa 488 (Thermo), where the mouse anti-T. gondii primary antibody was used, or with 1:1000 goat anti-mouse Alexa 488 (Thermo) and 1:1000 goat anti-rabbit Alexa 647 (Thermo) where the rabbit anti-T. gondii primary antibody was used. .. Images were acquired on a Nikon Microscopy:Article Title: High-throughput identification of pathogen effector proteins that target host transcription by dual perturb-seq Article Snippet: .. Images were acquired on a Nikon Article Title: GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies Article Snippet: .. Similarly to previously established protocols , experiments in Fig. and Supplementary Fig. , were analysed on a Nikon Article Title: A heterotrimeric complex of Toxoplasma proteins promotes parasite survival in interferon gamma-stimulated human cells. Article Snippet: .. Images were acquired on a Nikon Article Title: High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription. Article Snippet: Finally, the cells were strained with 5 mg/mL DAPI plus 1:1000 goat anti-mouse Alexa 647 (Thermo) and 1:1000 goat anti-rabbit Alexa 488 (Thermo), where the mouse anti-T. gondii primary antibody was used, or with 1:1000 goat anti-mouse Alexa 488 (Thermo) and 1:1000 goat anti-rabbit Alexa 647 (Thermo) where the rabbit anti-T. gondii primary antibody was used. .. Images were acquired on a Nikon Infection:Article Title: High-throughput identification of Toxoplasma gondii effector proteins that target host cell transcription. Article Snippet: Finally, the cells were strained with 5 mg/mL DAPI plus 1:1000 goat anti-mouse Alexa 647 (Thermo) and 1:1000 goat anti-rabbit Alexa 488 (Thermo), where the mouse anti-T. gondii primary antibody was used, or with 1:1000 goat anti-mouse Alexa 488 (Thermo) and 1:1000 goat anti-rabbit Alexa 647 (Thermo) where the rabbit anti-T. gondii primary antibody was used. .. Images were acquired on a Nikon |